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KMID : 0043319990220010055
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Archives of Pharmacal Research
1999 Volume.22 No. 1 p.55 ~ p.59
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Inhibition of Lipopolysaccaride-induced Inducible Nitric Oxide (iNOS) mRNA Expression and Nitric Oxide Production by Higenamine in Murine Peritoneal Macrophages
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Lee Hoi-Young
Lee Jang-Soon
Kim Eun-Ju
Han Jeung-Whan
Lee Hyang-Woo
Kang Young-Jin
Chang Ki-Churl
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Abstract
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Nitric oxide synthesized by inducible nitric oxide synthase (iNOS) has been implicated as a mediator of inflammation in rheumatic and autoimmune diseases. The effects of higenamine, a tetrahydroisoquinoline compound, on induction of NOS by bacterial lipopolysaccaride (LPS) were examined in murine peritoneal macrophages. LPS-induced nitrite/nitrate production was markedly inhibited by higenamine which at 0.01 mM, decreased nitrite/nitrate levels by This was comparable to the inhibition of LPS-induced nitrite/nitrate production by tetrandrin (). at the same concentration. Northern and Western blot analysis of iNOS expression demonstrated that iNOS expression was significantly attenuated following co-incubation of peritoneal macrophages with LPS (10 /m;; 18hrs) and higenamine (0.001, 0.,01 mM; 18hrs). These results suggest that higenamine can inhibit LPS-induced expression of iNOS mRNA in murine peritoneal macrophages. The clinical implications of these findings remain to be established.
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KEYWORD
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Higenamine, Inflammation, Nitric oxide, iNOS mRNA, Murine macrophages
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